A label-free biosensor for electrochemical detection of femtomolar microRNAs.
Identifieur interne : 000910 ( Main/Exploration ); précédent : 000909; suivant : 000911A label-free biosensor for electrochemical detection of femtomolar microRNAs.
Auteurs : RBID : pubmed:23323518English descriptors
- KwdEn :
- MESH :
- chemical , analysis : MicroRNAs.
- chemical , blood : Biological Markers, MicroRNAs.
- methods : Biosensing Techniques, Electrochemical Techniques.
- HeLa Cells, Humans.
Abstract
A simple and ultrasensitive label-free microRNA (miRNA) biosensor, based on hybridized miRNA-templated deposition of an insulating polymer film and electrochemical impedance spectroscopic detection, is described in this report. The biosensor is made of a monolayer of charge-neutral morpholino capture probes on an indium tin oxide (ITO)-coated glass slide. Upon hybridization, the neutral surface of the biosensor is converted to anionic by the hybridized miRNA strands. The deposition of the insulating polymer film, poly(3,3'-dimethoxybenzidine) (PDB), is then carried out by the horseradish peroxidase-catalyzed polymerization of 3,3'-dimethoxybenzidine in the presence of H(2)O(2). The cumulative nature of the PDB deposition process significantly enhances the sensitivity of the biosensor. Under optimized conditions, miRNA expression profiling can be performed label-freely from 5.0 fM to 2.0 pM with a detection limit of 2.0 fM. The biosensor is applied to the detection of circulating miRNAs in blood and miRNAs in total RNA extracted from cultured cells.
DOI: 10.1021/ac302883c
PubMed: 23323518
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The biosensor is made of a monolayer of charge-neutral morpholino capture probes on an indium tin oxide (ITO)-coated glass slide. Upon hybridization, the neutral surface of the biosensor is converted to anionic by the hybridized miRNA strands. The deposition of the insulating polymer film, poly(3,3'-dimethoxybenzidine) (PDB), is then carried out by the horseradish peroxidase-catalyzed polymerization of 3,3'-dimethoxybenzidine in the presence of H(2)O(2). The cumulative nature of the PDB deposition process significantly enhances the sensitivity of the biosensor. Under optimized conditions, miRNA expression profiling can be performed label-freely from 5.0 fM to 2.0 pM with a detection limit of 2.0 fM. The biosensor is applied to the detection of circulating miRNAs in blood and miRNAs in total RNA extracted from cultured cells.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23323518</PMID><DateCreated><Year>2013</Year><Month>02</Month><Day>05</Day></DateCreated><DateCompleted><Year>2014</Year><Month>04</Month><Day>22</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6882</ISSN><JournalIssue CitedMedium="Internet"><Volume>85</Volume><Issue>3</Issue><PubDate><Year>2013</Year><Month>Feb</Month><Day>5</Day></PubDate></JournalIssue><Title>Analytical chemistry</Title><ISOAbbreviation>Anal. Chem.</ISOAbbreviation></Journal><ArticleTitle>A label-free biosensor for electrochemical detection of femtomolar microRNAs.</ArticleTitle><Pagination><MedlinePgn>1624-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/ac302883c</ELocationID><Abstract><AbstractText>A simple and ultrasensitive label-free microRNA (miRNA) biosensor, based on hybridized miRNA-templated deposition of an insulating polymer film and electrochemical impedance spectroscopic detection, is described in this report. The biosensor is made of a monolayer of charge-neutral morpholino capture probes on an indium tin oxide (ITO)-coated glass slide. Upon hybridization, the neutral surface of the biosensor is converted to anionic by the hybridized miRNA strands. The deposition of the insulating polymer film, poly(3,3'-dimethoxybenzidine) (PDB), is then carried out by the horseradish peroxidase-catalyzed polymerization of 3,3'-dimethoxybenzidine in the presence of H(2)O(2). The cumulative nature of the PDB deposition process significantly enhances the sensitivity of the biosensor. Under optimized conditions, miRNA expression profiling can be performed label-freely from 5.0 fM to 2.0 pM with a detection limit of 2.0 fM. The biosensor is applied to the detection of circulating miRNAs in blood and miRNAs in total RNA extracted from cultured cells.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Zhiqiang</ForeName><Initials>Z</Initials><Affiliation>Department of Chemistry, National University of Singapore, Singapore 117543. chmgaoz@nus.edu.sg</Affiliation></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Huimin</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Yuqian</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>01</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Chem</MedlineTA><NlmUniqueID>0370536</NlmUniqueID><ISSNLinking>0003-2700</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Biological Markers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>MicroRNAs</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Biological Markers</DescriptorName><QualifierName MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrochemical Techniques</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">HeLa Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">MicroRNAs</DescriptorName><QualifierName MajorTopicYN="N">analysis</QualifierName><QualifierName MajorTopicYN="Y">blood</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2013</Year><Month>1</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>1</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>1</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>4</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1021/ac302883c</ArticleId><ArticleId IdType="pubmed">23323518</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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